| 中文名称 |
Sequencing Grade Modified Trypsin |
| 英文名称 |
Sequencing Grade Modified Trypsin |
Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Unmodified
trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC peptide analysis.
In addition, auto-proteolysis can result in the generation of pseudotrypsin, which has been shown to exhibit chymotrypsin-like
specificity (1). Promega Sequencing Grade Modified Trypsin is porcine trypsin modified by reductive methylation, rendering it
resistant to proteolytic digestion (2). In enzymatic stability tests, modified trypsin was found to retain greater than two times the
activity of unmodified trypsin.
